ISO/TS 20224-5:2020

Indisponible en français

Résumé

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of goat-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of goat material derived from goat (Capra hircus).

The target sequence is a partial fragment of the goat chromosome 9 DNA sequence (i.e. GenBank accession number NC_030816.1)^[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD_{95 %}).

Informations générales

État actuel
: Publiée

Date de publication
: 2020-07
Edition
: 1
Comité technique
: ISO/TC 34/SC 16 Méthodes horizontales pour l'analyse moléculaire de biomarqueurs
ICS
:
67.050 Méthodes générales d'analyse et d'essai des produits alimentaires